Repeated Administrations of Cardiac Progenitor Cells Are Markedly More Effective Than a Single Administration

Repeated Administrations of Cardiac Progenitor Cells Are Markedly More Effective Than a Single Administration

Repeated Administrations of Cardiac Progenitor Cells Are Markedly More Effective Than a Single Administration: A New Paradigm in Cell Therapy.

RATIONALE The effects of c-kit(POS) cardiac progenitor cells (CPCs, and adult cell therapy in general) on left ventricular (LV) function have been regarded as modest or inconsistent. OBJECTIVE To determine whether 3 CPC infusions have greater efficacy than 1 infusion. METHODS AND RESULTS Rats with a 30-day-old myocardial infarction received 1 or 3 CPC infusions into the LV cavity, 35 days a...

Repeated Administrations of Cardiac Progenitor Cells Are Superior to a Single Administration of an Equivalent Cumulative Dose

BACKGROUND We have recently found that 3 repeated doses (12×106 each) of c-kitPOS cardiac progenitor cells (CPCs) were markedly more effective than a single dose of 12×106 cells in alleviating postinfarction left ventricular dysfunction and remodeling. However, since the single-dose group received only one third of the total number of CPCs given to the multiple-dose group, it is unknown whether...

More than a cover: epicardium as a novel source of cardiac progenitor cells.

Bin Zhou1 & William T Pu2† †Author for correspondence 1Department of Cardiology, Children’s Hospital Boston and Harvard Stem Cell Institute, Harvard University, Boston, MA, USA Tel.: +1 617 919 4643; E-mail: [email protected]. harvard.edu 2Department of Cardiology, Children’s Hospital Boston and Harvard Stem Cell Institute, Harvard University, Boston, MA, USA Tel.: +1 617 919 2091; Fax: +1 617 7...

Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIThigh cells.

To clarify the phenotypes of various classes of human hematopoietic progenitor cells, we used a multicolor staining protocol in conjunction with CD34 and a newly developed mouse antihuman c-kit proto-oncogene product (KIT) monoclonal antibody (MoAb). We characterized three cell fractions in CD34+ cells that express KITlow and KIThigh cells in addition to KIT- cells. A clonogenic assay showed th...